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>   home   >   Products   >   Primary Antibodies   >   Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody   

Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody AG1422-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1-2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody (#AG1422), (1:400).
    3-4. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody AG1422-025
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
    1. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody (#AG1422), (1:200).
    2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody AG1422-025
    Expression of nAChRα2 in rat deep cerebellar nucleus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody (#AG1422), (1:100). Staining reveals expression of nAChRα2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
  • ICC - Nicotinic Acetylcholine Receptor Alpha2 (extracellular) Antibody AG1422-025
    Expression of Nicotinic Acetylcholine Receptor α2 in rat PC12 pheochromocytoma cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells.  A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) antibody (#AG1422), (1:50), followed by goat anti-rabbit-AlexaFluor-594  (red). B. Live image of the cells. C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12389
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 58611 Da
Homology Mouse - identical; human - 12/13 amino acid residues identical.
Additional Information
Gene ID 170945
Other Names Neuronal acetylcholine receptor subunit alpha-2, Chrna2, Acra2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChR־±2 (Accession P12389). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Nicotinic Acetylcholine Receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and have longer-term effects on gene expression and cellular connections1.   nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits (‘structural’ subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2.  There are nine α subunits (α2–α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and and β (2-4) subunits.    All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5.   In humans, a mutant α2 subunit has been identified, which forms nAChRs with increased agonist sensitivity and causes a form of familial epilepsy6. Further, an α2 subunit null mouse model has been used to demonstrate a role for α2 nAChR in nicotine-induced modulation of long-term potentiation in the mouse hippocampal CA1 region, which may underlie some of the cognitive effects of nicotine7.


References 1. Jensen, A.A. et al.  (2005)  J. Med. Chem. 48, 4705. 2. Millar, N.S. and Harkness, P.C. (2008) Mol. Membr. Biol. 25, 279. 3. Hogg, R.C. et al. (2003) Rev. Physiol. Biochem. Pharmacol. 147,1. 4. Fu, X.W. et al. (2009) Am. J. Respir. Cell. Mol. Biol. 41, 93. 5. De Rosa, M.J. et al. (2009) Life Sci. 85, 449. 6. Aridon, P. et al. (2006) Am. J. Human Genetics 79, 342. 7. Nakauchi, S. et al. (2007) Eur. J. Neurosci. 25, 2666.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1422-025
(40 western blots)
Availability: 2-3 weeks
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