|Application ||WB, LCI|
|Calculated MW||78036 Da|
|Homology||Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.|
|Other Names||Lutropin-choriogonadotropic hormone receptor, LH/CG-R, Luteinizing hormone receptor, LSH-R, Lhcgr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide ENELSGWDYDYGFC, corresponding to amino acid residues 327-340 of rat LH receptor (Accession P16235). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The luteinizing hormone receptor (LHR) is a member of the subfamily of glycoprotein hormone receptors within the superfamily of G-protein coupled receptor (GPCR)/seven-transmembrane domain receptors1. The LH/CG receptor is comprised of two structurally and functionally distinct domains, extracellular N-terminal exodomain and membrane-embedded endodomain. These two domains can separately be expressed and processed, including folding. The exodomain alone has the high-affinity hormone binding site but is not capable of generating hormonal signal. In contrast, the endodomain alone has the site for receptor activation2. For many years, LHR was thought to be localized strictly to gonadal cells. In the testes, the LHR is thought to be restricted to Leydig cells. In the ovary, expression of the LHR occurs in theca cells, interstitial cells, differentiated granulosa cells, and luteal cells3. There is increasing evidence that LHR may be present in extragonadal tissues such as: uterus, female reproductive tract tissues, sperm, seminal vesicles, prostate, skin, breast cell lines, lactating mammary gland, adrenals, neural retina, neuroendocrine cells, and brain4. Mutations in the LH receptor have a strong impact on the male phenotype. Activating mutations of the LH receptor leads to male-limited pseudoprecocious puberty, whereas inactivating mutations result in male pseudohermaphroditism5.
References 1. Dufau, M.L. et al. (1998) Annu. Rev. Physiol. 60, 461. 2. Ji, T.H. et al. (1997) Recent Prog. Horm. Res. 52, 431. 3. Ascoli, M. et al. (2002) Endocr. Rev. 23, 141. 4. Rao, C.V. et al. (1996) J. Physiol. Pharmacol. 47, 41. 5. Simoni, M. et al. (1997) Endocr. Rev. 18, 739.
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