|Reactivity||Human, Mouse, Rat|
|Calculated MW||120628 Da|
|Homology||Mouse, human - identical.|
|Other Names||Solute carrier family 12 member 4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, rKCC1, Furosemide-sensitive K-Cl cotransporter, Slc12a4, Kcc1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HAPDNFRELVHIK, corresponding to amino acid residues 998- 1010 of rat KCC1 (Accession Q63632). Intracellular, C-terminus.Several splice variants of the KCC1 human gene have been identified. This antibody will recognize all known variants except for isoform 4. For more information go to human KCC1 (Accession Q9UP95).ֲ|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Potassium chloride cotransporters (KCCs) are members of cation chloride cotransporter gene superfamily (Slc12 family). This family consists of sodium potassium two chloride cotransporter 1 (NKCC1) and 2 (NKCC2), sodium chloride cotransporte (NCC) and KCCs1. All of these transporters mediate electroneutral transport of cations coupled with one or two Cl- and contribute to regulation of cell volume and homeostasis of intracellular Cl- concentration. KCCs extrude one K+ with one Cl- from intracellular space to the extracellular space by using the chemical gradient of K+ as the driving force of Cl- extrusion2. Four KCC isofroms (KCC1, KCC2, KCC3 and KCC4) have been identified in mammalian species 3. K-Cl cotransporters regulate neuronal and glial electrochemical equilibrium potential for Cl-, and so can determine the excitatory or inhibitory influences of GABA- and glycine-gated Cl- channels4. KCC1 is a member of the cation-chloride cotransporter (CCC) superfamily of proteins, as with all CCC proteins, KCC1 is an integral membrane protein with 12 transmembrane domains and both N- and C-terminal cytoplasmic domains 5. KCC1 is ubiquitously expressed6. K-Cl cotransport activity is elevated in red blood cells of individuals with sickle cell disease, thalassemia, and diseases associated with other mutant Hb. Human erythrocytes from individuals with the reticulocytotic anemic states of sickle cell disease, Hb SC disease, and β-thalassemia intermedia exhibit uniformly elevated KCC1-like immunoreactivity compared with normal AA erythrocytes 7.
References 1. Russell J.M. (2000) Physiol. Rev. 80, 211. 2. Nagao, H. et al. (2012) Cell. Physiol. Biochem. 30, 538. 3. Gamba, G. (2005) Physiol. Rev. 85, 423. 4. Delpire, E. and Gagnon, K.B. (2008) Biochem. J. 409, 321. 5. Lee, H.H. et al. (2007) J. Biol. Chem. 282, 29777. 6. Gillen, C.M. et al. (1996) J. Biol. Chem. 271, 16237. 7. Olivieri, O. et al. (1992) Blood 79, 793.
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