|Calculated MW||42586 Da|
|Homology||Rat - 13/14 amino acid residues identical; mouse - 12/14 amino acid residues identical.|
|Other Names||KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, hOT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, KISS1R, AXOR12, GPR54|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R (Accession Q969F8). 3rd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||4 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The Kisspeptin receptor (KISS1R, also named GPCR54) is a member of the G-protein coupled receptor superfamily. The KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey’s scientists who named it after their famous chocolate, the “Kiss”. The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis. KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found within the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes. 1-3 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human Kisspeptin receptor (KISS1R). Anti-Kisspeptin Receptor (extracellular) antibody (#AG1426) can be used for Western blot analysis and immunohistochemical applications. It has been designed to recognize KISS1R from human, rat and mouse samples.
References 1. IUPHAR DATABASE | KiSS1-derived peptide receptor. 2. Kauffman, A.S. et al. (2007) J. Neurosci. 27, 8826. 3. Dungan, H.M. et al. (2006) Endocrinology 147, 1154.
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