|Calculated MW||52617 Da|
|Homology||Mouse- identical; human - 12/13 amino acid residues identical.|
|Other Names||Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, Glra1, Glyr|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyR־±1(Accession P07727). 2nd intracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Glycine receptors (GlyRs) mediate ionotropic inhibitory neurotransmission in the CNS where they play an essential role in inhibition of motor neurons in the spinal cord and brainstem1. The glycine receptor is composed of three ligand-binding subunits of 48 kDa (α) and two homologous polypeptides of 58 kDa (β) that span the postsynaptic membrane in a pentameric arrangement to form a Cl--selective channel2. So far, at least three different α-subunit variants of the GlyR have been identified: α1, α2 and α33. Homo-oligomeric α2 receptors have a low strychnine-binding affinity and are considered to represent a neonatal form of GlyR, which is replaced during development by receptors containing the α1 subunit. Adult GlyRs are predominantly composed of α1 and β subunits, the α3 subunit being expressed at low levels in only a few brain regions4. GlyRα1 subunits are widely expressed and contribute to many processes in the CNS, including inflammatory pain perception, modulation of auditory and visual pathways, and neurotransmission in the cerebellar cortex5. Mutations in glycine receptor α1 subunits cause human hereditary hyperekplexia, a disorder characterized by an exaggerated startle response, which is a consequence of the role of GlyRs in motor reflex circuits of the spinal cord6.
References 1. Werman, R. et al. (1967) Nature 214, 681. 2. Betz, H. et al. (1992) Q. Rev. Biophys .25, 381. 3. Kuhse, J. et al. (1990) J. Biol. Chem. 265, 223. 4. Malosio, M.L. et al. (1991) Embo J. 10, 2401. 5. Durisic, N. et al. (2012) J. Neurosci. 32, 12915. 6. Bakker, M.J. et al. (2006) Lancet Neurol. 5, 513.
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