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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   AMPA Receptor 2 (GluR2) (extracellular) Antibody   

AMPA Receptor 2 (GluR2) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - AMPA Receptor 2 (GluR2) (extracellular) Antibody AG1429-025
    Western blot analysis of rat brain membranes:
    1. Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody (#AG1429), (1:200).
    2. Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody, preincubated with the control peptide antigen.
  • IP - AMPA Receptor 2 (GluR2) (extracellular) Antibody AG1429-025
    Immunoprecipitation of rat brain lysates: 1. Brain lysates + protein A beads + Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody.
    2. Brain lysates + protein A beads + pre-immune rabbit serum.
    3. Brain lysates.

    Black arrow indicates the AMPA Receptor 2 (GluR2) protein while the red arrow shows the IgG heavy chain. The immunoblot was performed with Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody (#AG1429).
  • IHC - AMPA Receptor 2 (GluR2) (extracellular) Antibody AG1429-025
    Expression of AMPA Receptor 2 in rat cerebellum
    Immnunohistochemical staining of rat cerebellum using Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody (#AG1429) . A. Co-localization of AMPA Receptor 2 (green) and calbindin D28-K (red) in Purkinje cell dendrites (vertical arrow). B. Distribution of calbindin D28-K. C. Distribution of AMPA Receptor 2. Note that some AMPA Receptor 2 positive fibers (horizontal arrow) that are probable Bergmann glial processes do not stain for AMPA Receptor 2. D. The molecular and Purkinje layer (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19491
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 98688 Da
Homology Mouse - identical; human - 14/15 amino acid residues identical.
Additional Information
Gene ID 29627
Other Names Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic, AMPA 2, GluA2, Gria2, Glur2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide NVGNINNDKKDETYR(C), corresponding to amino acid residuesֲ 179-193 of rat AMPA Receptor 2 (Accession P19491). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Rat cortical lysate (1:5000) (see Banerjee, B. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Research Areas
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AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.   The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.   The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.   AMPA receptors are permeable to cations Na+, K+ and Ca2+.  The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of the amino acid arginine (R) at a critical site in the pore loop instead of glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.   Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.   Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.   Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.


References 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. 2. Song, I. and Huganir, R.L. (2002) Trends Neurosci. 25, 578. 3. Malinow, R. and Malenka, R.C. (2002) Annu. Rev. Neurosci. 25, 103.

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$ 375.00
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Cat# AG1429-025
(40 western blots)
Availability: 2-3 weeks
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