|Reactivity||Human, Mouse, Rat|
|Calculated MW||72799 Da|
|Homology||Rat, human - identical.|
|Other Names||Gamma-aminobutyric acid receptor subunit theta, GABA(A) receptor subunit theta, Gabrq|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABA(A) ־¸ Receptor (Accession Q9JLF1). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The A-type receptors for γ-aminobutyric acid (GABA(A)) mediate the majority of fast inhibitory transmission in the mammalian central nervous system. Many clinically important drugs including benzodiazepines, barbiturates and general anesthetics act as positive allosteric modulators of this receptor1. GABA(A) receptors are pentameric ligand-gated ion-channels, composed of subunits from many subunit classes: α1-α6, β1-β4, γ1-γ4, δ, ε , θ, π and ρ1-ρ32. The arrangement of subunits around the channel is probably γβαβα counter-clockwise when viewed from the extracellular space, ε and π subunits can replace the γ and δ subunit within the pentamer, whereas the θ subunit could replace a β subunit3. The localized expression of this subunit is consistent with the fact that all GABA(A) receptor subunit genes have distinct expression patterns in the brain3. The θ subunit has been reported to be strongly expressed in the striatum and has been localized to the locus ceruleus4. Impairment of the γ-aminobutyric acid (GABA) signaling system is believed to partially account for behavioral and cognitive deficits associated with schizophrenia and mood disorders5. Reduction of GABA(A) mediated signal transmission has also been associated with anxiety, panic, impaired learning and memory6.
References 1. Hevers, W. and Luddens, H. (1998) Mol. Neurobiol. 18, 35. 2. Barnard, E.A. et al. (1998) Pharmacol. Rev. 50, 291. 3. Sieghart, W. and Sperk, G. (2002) Curr. Top. Med. Chem. 2, 795. 4. Wisden, W. et al. (1992) J. Neurosci. 12, 1040. 5. Bonnert, T.P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 9891. 6. Pinna, G. et al. (2006) Psychopharmacology (Berl) 186, 362.
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