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FSH Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - FSH Receptor (extracellular) Antibody AG1431-025
    Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 (lane 3 and 6) lysates:
    1-3. Anti-FSH Receptor (extracellular) antibody (#AG1431), (1:200).
    4-6. Anti-FSH Receptor (extracellular) antibody, preincubated with the control antigen.
  • ICC - FSH Receptor (extracellular) Antibody AG1431-025
    Expression of FSH receptor in human ovary cell line
    Immunocytochemical staining of intact living human OVCAR3 cells. A. Extracellular staining of cells using Anti-FSH Receptor (extracellular) antibody (#AG1431), (1:25) followed by goat anti-rabbit AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20395
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 77681 Da
Homology Mouse, human - 12/13 amino acid residues identical.
Additional Information
Gene ID 25449
Other Names Follicle-stimulating hormone receptor, FSH-R, Follitropin receptor, Fshr
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSH Receptor (Accession P20395). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin. FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4. Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1431-025
(40 western blots)
Availability: 2-3 weeks
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