|Application ||WB, IHC|
|Calculated MW||49428 Da|
|Homology||Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.|
|Other Names||D(1A) dopamine receptor, Dopamine D1 receptor, Drd1, Drd1a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat D1 Dopamine Receptor (Accession P18901). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The D1 Dopamine Receptor (D1 Receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia1. All five dopamine receptors belong to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1. The D1-like Receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like Receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1. The D1 Receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 Receptor has been detected in the adrenal cortex, kidney and heart. Functionally, the D1 Receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways. In addition, D1 Receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat D1 Receptor. Anti-D1 Dopamine Receptor antibody (#AG1433) can be used in western blot analysis and immunohistochemical applications, and recognizes D1 Receptor from rat and mouse samples.
1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
2. Zeng, C. et al. (2008) Am. J. Physiol. 294, H551.
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