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D1 Dopamine Receptor Antibody

Affinity purified rabbit polyclonal antibody

     
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  • WB - D1 Dopamine Receptor Antibody AG1433-025
    Western blot analysis of lysates from human brain, rat testis, rat brain and mouse brain tissue lysate(from left to right), using Drd1 Antibody(Cat. #AG1433). AG1433 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - D1 Dopamine Receptor Antibody AG1433-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1, 2. Anti-D1 Dopamine Receptor antibody (#AG1433), (1:200). 3, 4. Anti-D1 Dopamine Receptor antibody, preincubated with the control peptide antigen.
  • IHC - D1 Dopamine Receptor Antibody AG1433-025
    Expression of D1 Dopamine Receptor in rat cortex Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor antibody (#AG1433), (1:100), (green). A. D1 Dopamine Receptor appears in the soma and dendrites of cortical pyramidal neurons in layer 5. B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of D1 Dopamine Receptor is restricted to pyramidal neurons. DAPI is used as the counterstain.
  • IHC - D1 Dopamine Receptor Antibody AG1433-025
    Expression of D1 Dopamine Receptor in rat striatum Immunohistochemical staining of perfusion-fixed frozen brain sections with Anti-D1 Dopamine Receptor antibody (#AG1433), (1:100), (green). A. D1 Dopamine Receptor appears in the striatal matrix (star). B. The same section was stained for Calbindin D28k (red), a marker of interneurons. C. Merging of the two images demonstrates that localization of D1 Dopamine Receptor is restricted to the matrix. DAPI is used as the counterstain.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P18901
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 49428 Da
Homology Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.
Additional Information
Gene ID 24316
Other Names D(1A) dopamine receptor, Dopamine D1 receptor, Drd1, Drd1a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat D1 Dopamine Receptor (Accession P18901). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Background

The D1 Dopamine Receptor (D1 Receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia1. All five dopamine receptors belong to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1. The D1-like Receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like Receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1. The D1 Receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 Receptor has been detected in the adrenal cortex, kidney and heart. Functionally, the D1 Receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways. In addition, D1 Receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat D1 Receptor. Anti-D1 Dopamine Receptor antibody (#AG1433) can be used in western blot analysis and immunohistochemical applications, and recognizes D1 Receptor from rat and mouse samples.

References

1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
2. Zeng, C. et al. (2008) Am. J. Physiol. 294, H551.

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Cat# AG1433-025
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