|Application ||WB, FC, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||120674 Da|
|Homology||Rat, mouse, bovine, canis - identical.|
|Other Names||Extracellular calcium-sensing receptor, CaSR, Parathyroid cell calcium-sensing receptor 1, PCaR1, CASR, GPRC2A, PCAR1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180). N-terminus, Extracellular.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The Calcium-Sensing Receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism, such as parathyroid cells and kidney.1,2 The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3 CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4 CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5 Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas,lower levels of expression were found in compared to normal colon tissue. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope at the N-terminus of human CaSR. Anti-Calcium Sensing Receptor (extracellular) antibody (#AG1435) can be used in western blot analysis and immunocytochemistry applications, and recognizes CaSR in human, rat and mouse samples.
1. IUPHAR RECEPTOR DATABASE.
2. Racz, G.Z. et al. (2002) Gut 51, 705.
3. Canaff, L. and Hendy, G.N. (2002) J. Biol. Chem. 277, 30337.
4. Chen, R.A. and Goodman, W.G. (2004) Am. J. Physiol. Renal Physiol. 286, F1005
5. Chattopadhyay, N. (2006) Am. J. Physiol. Endocrinol. Metab. 290, E761.
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