|Calculated MW||110916 Da|
|Homology||Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.|
|Other Names||Anoctamin-1, Transmembrane protein 16A, Ano1, Tmem16a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (Accession Q8BHY3). 3rd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Anoctamin (ANO, or TMEM16) is a family of membrane proteins which includes 10 members. This family is named so because these channels selective to ANions and have eight (OCT) transmembrane domains. Also, these channels are subject to glycosylation in their extracellular loops and have both intracellular N- and C-termini1. Members of this family are expressed in a broad range of different organisms ranging from mammals, flies, worms, plants as well as yeast1. Alternative splicing is known to affect these channels and regarding their oligomerization state, homedimerization has been observed although when heterologously expressed, these channels may hetero oligomerize2. Ano1 (or TMEM16A, DOG1 and others) the first member to be identified was found to be a Ca2+-activated Cl- channel3-5 therefore other members are likely to also be Cl- channels. These channels are expressed in many different tissues: bronchiolar epithelial cells, pancreatic acinar cells, proximal kidney tubule epithelium, retina, dorsal root ganglia and submandibular gland1. In fact, Ano1 gained a lot of attention as its activation may serve as a therapeutic treatment for cystic fibrosis since it is also expressed in the airways6. These Ca2+-activated Cl- channels are believed to play a role in development as knock out of Ano1 in mice causes abnormal development of the trachea7. Ano2 (TMEM16B) has been shown to mediate Ca2+-activated Cl- current in olfactory epithelium and photoreceptor synapses2,8,9. Although relatively newly discovered channels, they are being discovered in many medical indications. Ano1 has become a marker in gastrointestinal tumors as its expression is significantly upregulated in such tumors10,11. Similarly, Ano1 is also highly expressed other carcinomas12,13. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Anoctamin 1 channel. Anti-Anoctamin-1 (extracellular) antibody (#AG1437) can be used in western blot analysis and has been designed to recognize Anoctamin 1 channel from mouse, rat and human samples.
References 1. Hartzell, H.C. et al. (2009) J. Physiol. 587.10, 2127. 2. Sheridan, J.T. et al. (2011) J. Biol. Chem. 286, 1381. 3. Caputo, A. et al. (2008) Science 322, 590. 4. Yang, Y.D. et al. (2008) Nature 455, 1210. 5. Schroeder. B.C. et al. (2008) Cell 134, 1019. 6. Rock, J.R. et al. (2009) J. Biol. Chem. 284, 14875. 7. Rock, J.R. et al. (2008) Dev. Biol. 321, 141. 8. Stephan, A.B. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 11776. 9. Hengl, T. et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 6052. 10. West, R.B. et al. (2004) Am. J. Pathol. 165, 107. 11. Espinosa, I. et al. (2008) Am. J. Surg. Pathol. 32, 210. 12. Huang, X. et al. (2006) Genes Chromosomes Cancer 45, 1058. 13. Carles, A. et al. (2006) Oncogene 25, 1821.
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