|Calculated MW||51130 Da|
|Homology||Rat - identical; human -15/16 amino acid residues identical.|
|Other Names||Cation channel sperm-associated protein 4, CatSper4, Catsper4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4 (Accession Q8BVN3). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2. CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence T×D×W indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH- and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6. Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.
References 1. Yanagimachi, R. et al. (1994) The Physiology of Reproduction (Raven Press, New York) 2nd edition, 189. 2. Jin, J.L. et al. (2005) Biol. Reprod. 73, 1235. 3. Quill, T.A. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 12527. 4. Ren, D. et al. (2001) Nature 413, 603. 5. Kirichok, Y. et al. (2006) Nature 439, 737. 6. Lobley, A. et al. (2003) Reprod. Biol. Endocrinol. 1, 53. 7. Carlson, A.E. et al. (2005) J. Biol. Chem. 280, 32238.
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