|Application ||WB, LCI|
|Calculated MW||98010 Da|
|Homology||Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Trpv4, Vroac|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65. TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat TRPV4. Anti-TRPV4 (extracellular) antibody (#AG1440) can be used in western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize TRPV4 from human, rat and mouse samples.
References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.
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