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>   home   >   Products   >   Primary Antibodies   >   TRPV4 (extracellular) Antibody   

TRPV4 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPV4 (extracellular) Antibody AG1440-025
    Western blot analysis of rat brain lysate: 1. Anti-TRPV4 (extracellular) antibody (#AG1440), (1:200).
    2. Anti-TRPV4 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - TRPV4 (extracellular) Antibody AG1440-025
    Expression of TRPV4 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) antibody (# AG1440). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.
  • ICC - TRPV4 (extracellular) Antibody AG1440-025
    Expression of TRPV4 in human U-87 cells Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using  Anti-TRPV4 (extracellular) antibody (#AG1440), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.  DAPI was used as the counterstaining (blue).
  • ICC - TRPV4 (extracellular) Antibody AG1440-025
    Expression of TRPV4 in human U-87 cells Immunocytochemical staining of live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) antibody (#AG1440), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9ERZ8
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 98010 Da
Homology Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.
Additional Information
Gene ID 66026
Other Names Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Trpv4, Vroac
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65. TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat TRPV4. Anti-TRPV4 (extracellular) antibody (#AG1440) can be used in western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize TRPV4 from human, rat and mouse samples.


References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1440-025
(40 western blots)
Availability: 2-3 weeks
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