|Calculated MW||307058 Da|
|Homology||Mouse, human - 13/14 amino acid residues identical.|
|Other Names||Inositol 1, 5-trisphosphate receptor type 2, IP3 receptor isoform 2, IP3R 2, InsP3R2, Type 2 inositol 1, 5-trisphosphate receptor, Type 2 InsP3 receptor, Itpr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2 (Accession P29995).|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Inositol 1,4,5-trisphosphate receptors (IP3Rs) are intracellular Ca2+ release channels located on the endoplasmic reticulum (ER) and mediate Ca2+ mobilization from the ER to the cytoplasm in response to the binding of the second messenger, inositol 1,4,5-trisphosphate (IP3)1. IP3-induced Ca2+ release is triggered by various external stimuli, and most non-excitable cells use this mechanism as the primary Ca2+ signaling pathway. IP3Rs are therefore thought to have important physiological roles in various cell types and tissues2. Three subtypes of IP3Rs, derived from three distinct genes, have been identified in mammals3. All three receptors have six transmembrane domains and a pore domain between TM5 and TM6. The N-terminus as well as the C-terminus are cytoplasmic. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required to maintain the suppression of channel activity4. Type 2 IP3R (IP3R2) is expressed in various tissues and cell lines. IP3R2 mRNA is localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary5. IP3R2 is active during osteoclast differentiation, and its absence causes a partial defect in osteoclastic differentiation.
References 1. Furuichi, T. et al. (1989) Nature 342, 32 2. Berridge, M.J. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 517. 3. Furuichi, T. et al. (1994) Curr. Opin. Neurobiol. 4, 294. 4. Varnai, P. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 7859. 5. Fujino, I. et al. (1995) Cell Tissue Res. 280, 201. 6. Yaroslavskiy, B.B. et al. (2007) J. Cell Sci. 120, 2884.
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