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>   home   >   Products   >   Primary Antibodies   >   CaVGamma5 (extracellular) Antibody   

CaVGamma5 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - CaVGamma5 (extracellular) Antibody AG1442-025
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1, 2. Anti-CaVγ5 (extracellular) antibody (#AG1442), (1:1000).
    3, 4. Anti-CaVγ5 (extracellular) antibody preincubated with the control peptide antigen.
  • ICC - CaVGamma5 (extracellular) Antibody AG1442-025
    Expression of CaVγ5 in living rat PC12 cells Immunocytochemical staining of intact living rat PC12 cells using Anti-CaVγ5 (extracellular) antibody (#AG1442), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue). 
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8VHW8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 30924 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 140726
Other Names Voltage-dependent calcium channel gamma-5 subunit, Neuronal voltage-gated calcium channel gamma-5 subunit, Transmembrane AMPAR regulatory protein gamma-5, TARP gamma-5, Cacng5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)SESTVNVLKMIRS, corresponding to amino acid residues 87-99 of rat Cav־³5 (Accession Q8VHW8). 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.   CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.   The CaVγ5 subunit is highly expressed in the liver, kidney, heart, lung, skeletal muscles, and, with a lower abundance, in testes5. The close association of epilepsy and ataxia with mutations in other neuronal voltage-dependent Ca2+ channels suggests these are potential candidate phenotypes for defects in the CaVγ5 (CACNG5) gene6.


References 1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.  2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.  3. De Jongh, K.S. et al. (1990)   J. Biol. Chem. 265, 14738. 4. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298. 5. Lacinova, L. et al. (2005) Gen. Physiol. Biophys. 1, 1. 6. Klugbauer N. et al. (2000) FEBS Lett. 470,189. 7. Burgess, D.L. et al. (2009) Genome Res. 9, 1204.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1442-025
(40 western blots)
Availability: 2-3 weeks
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