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CaVGamma4 Antibody

Affinity purified polyclonal antibody

  • WB - CaVGamma4 Antibody AG1443-025
    Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), SH-SY5Y (lanes 3 and 7) and PC-12 (lanes 4 and 8) lysates: 1-4. Anti-Cavγ4 antibody (#AG1443), (1:800).
    5-8. Anti-Cavγ4 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8VHW9
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 36523 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 140725
Other Names Voltage-dependent calcium channel gamma-4 subunit, Neuronal voltage-gated calcium channel gamma-4 subunit, Transmembrane AMPAR regulatory protein gamma-4, TARP gamma-4, Cacng4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EKNKELRFKTKRE, corresponding to amino acid residues 208-220 of rat Cav־³4 (Accession Q8VHW9). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation1. They are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type2.   L-type calcium channels are composed of five subunits. Cavγ4, represents one of these subunits, and is one of several γ subunit proteins. It is an integral membrane protein that is thought to stabilize the Ca2+ channel in an inactive (closed) state. The encoding gene is a member of the neuronal Ca2+ channel γ subunit gene subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two similar gamma subunit-encoding genes3.   Cavγ4, along with Cavγ2, Cavγ3 and Cavγ8 have been associated with AMPA receptors and have been shown to modulate the trafficking of receptors to the plasma membrane as well as electrophysiological key properties4.


References 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214. 2. Scheuber, A. et al. (2004) J. Neurosci. 24, 10402. 3. Burgess, D.L. et al. (1999) Genome Res. 9, 1204. 4. Korber, C. et al. (2007) J. Neurosci. 27, 8442.

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$ 375.00
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$ 575.00
Cat# AG1443-025
(40 western blots)
Availability: 2-3 weeks
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