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CaVgamma3 Antibody

Affinity purified polyclonal antibody

  • WB - CaVgamma3 Antibody AG1444-025
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cells (lanes 3 and 6): 1-3. Anti-CaVγ3 antibody (#AG1444), (1:1000).
    4-6. Anti-CaVγ3 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8VHX0
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 35516 Da
Homology Mouse - identical, human - 12/14 amino acid residues identical.
Additional Information
Gene ID 140724
Other Names Voltage-dependent calcium channel gamma-3 subunit, Neuronal voltage-gated calcium channel gamma-3 subunit, Transmembrane AMPAR regulatory protein gamma-3, TARP gamma-3, Cacng3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RSHSELLKKSTFAR, corresponding to amino acid residues 210-223 of rat CaV־³3 (Accession Q8VHX0). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P/Q, and R types2.   L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the subunits α2δ, γ, and β3.   The γ subunit is an integral membrane protein. The γ family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5. CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. CaVγ3 mRNAs are only detectable in mouse brain6.


References 1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521. 2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485. 3. De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738. 4. Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298. 5. Lacinova, L. et al. (2005) Gen. Physiol. Biophys. Suppl.1, 1. 6. Klugbauer N. et al. (2000) FEBS Lett. 470, 189.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1444-025
(40 western blots)
Availability: 2-3 weeks
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