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CaVAlpha2Delta3 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - CaVAlpha2Delta3 (extracellular) Antibody AG1446-025
    Western blot analysis of  rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates:
    1, 2. Anti-CaVα2δ3 (extracellular) antibody (#AG1446), (1:200).
    3, 4. Anti-CaVα2δ3 (extracellular) antibody preincubated with the control peptide antigen.
  • IHC - CaVAlpha2Delta3 (extracellular) Antibody AG1446-025
    Expression of Cavα2δ3 in rat hippocampus and cortex Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-Cavα2δ3 (extracellular) antibody (#AG1446). In both areas Cavα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).
  • ICC - CaVAlpha2Delta3 (extracellular) Antibody AG1446-025
    Expression of CaVα2δ3 in rat PC12 cells
    Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells using Anti-Cavα2δ3 (extracellular) antibody (#AG1446), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8CFG5
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 122204 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 306243
Other Names Voltage-dependent calcium channel subunit alpha-2/delta-3, Voltage-gated calcium channel subunit alpha-2/delta-3, Voltage-dependent calcium channel subunit alpha-2-3, Voltage-dependent calcium channel subunit delta-3, Cacna2d3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaV־±2־´3 (Accession Q8CFG5). Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, CaV channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. HVA channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.  The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5. There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. The CaVα2δ3 subunit is predominantly expressed in neuronal tissue. The CaVα2δ3 subunit regulates all classes of HVA calcium channels. The Caα2δ3 subunits in the nerve terminal function in synaptic morphogenesis and cytoskeletal organization, and that this role is independent of their function in α1 subunit localization and physiology. CaVα2δ3 is likely to be the primary presynaptic α2δ isoform mediating morphological development of the neuromuscular junction (NMJ), since null alleles have such a large effect on NMJ development and abolish all action-potential evoked transmission7. Recent study shows that methylation-dependent transcriptional silencing of CaVα2δ3 may contribute to the metastatic phenotype of breast cancer8.


References 1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.  2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.   3. De Jongh, K.S. et al. (1990)   J. Biol. Chem. 265, 14738.  4. Sipos, I. et al. (2000) Pflug. Arch. 439, 691.  5. Dolphin, A.C. (2009) Curr. Opin. Neurobiol. 19, 237.  6. Cooper C.L. et al. (1987) J. Biol. Chem. 262, 509.  7. Kurshan P.T. (2009) Nat. Neurosci. 12, 1415. 8. Palmieri, C. et al. (2012) Br. J. Cancer 108, 375.

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Cat# AG1446-025
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