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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   STIM2 Antibody   

STIM2 Antibody

Affinity purified polyclonal antibody

  • WB - STIM2 Antibody AG1447-025
    Western blot analysis of rat brain (lanes 1,3) and RBL cell (lanes 2,4) lysates:
    1. Anti-STIM2 antibody (#AG1447), (1:200).
    2. Anti-STIM2 antibody, preincubated with the control peptide antigen.
  • IHC - STIM2 Antibody AG1447-025
    Expression of STIM2 in rat lung
    Immunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 antibody (#AG1447), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.  
  • ICC - STIM2 Antibody AG1447-025
    Expression of STIM2 in RBL cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat basophilic leukemia (RBL) cells.
    A, D. Cells stained with Anti-STIM2 antibody (#AG1447), (1:100) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
    B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.
    C. Merged image of panels A and B
    F. Merged image of panels D and E.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P246
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 83971 Da
Homology Rat, mouse, bovine - identical.
Additional Information
Gene ID 57620
Other Names Stromal interaction molecule 2, STIM2, KIAA1482
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research. Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5  The function of STIM2 still remains elusive and several models have been suggested.6-8   Abgent is pleased to offer a highly specific antibody directed against human STIM2. Anti-STIM2 antibody (#AG1447) can be used in western blot, immunocytochemical, immunohistochemical applications. It has been designed to recognize STIM2 from human, rat, mouse and bovine samples.


References 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. 5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123. 6. Parvez, S. et al. (2007) FASEB J.  22, 752. 7. Brandman, O. et al. (2007) Cell 131, 1327. 8. Soboloff, J. et al. (2006) Curr. Biol. 16, 1465.

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Cat# AG1447-025
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