|Calculated MW||83971 Da|
|Homology||Rat, mouse, bovine - identical.|
|Other Names||Stromal interaction molecule 2, STIM2, KIAA1482|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research. Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8 Abgent is pleased to offer a highly specific antibody directed against human STIM2. Anti-STIM2 antibody (#AG1447) can be used in western blot, immunocytochemical, immunohistochemical applications. It has been designed to recognize STIM2 from human, rat, mouse and bovine samples.
References 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. 3. Feske, S. et al. (2006) Nature 441, 179. 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. 5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123. 6. Parvez, S. et al. (2007) FASEB J. 22, 752. 7. Brandman, O. et al. (2007) Cell 131, 1327. 8. Soboloff, J. et al. (2006) Curr. Biol. 16, 1465.
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