|Reactivity||Human, Mouse, Rat|
|Calculated MW||212697 Da|
|Homology||Rat, mouse- identical.|
|Other Names||Transient receptor potential cation channel subfamily M member 7, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, TRPM7, CHAK1, LTRPC7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CKRRKKDKTSDGPKLFLTEE, corresponding to amino acidֲ residues 1146-1165 of human TRPM7 (Accession Q96QT4). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4 The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5 TRPM7 and TRPM6 are involved in Mg2+ homeostasis and are unique among the TRP family members, possessing a functional kinase domain at their C-terminus.6,7 Although the kinase is not necessary to the function of the channel it may have a role in modulating the activation of the channel.7 Recent work demonstrated that TRPM7 is a critical mediator of anoxic cell death.7,8 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of human TRPM7. The Anti-TRPM7 antibody (#AG1448) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize TRPM7 from human, rat and mouse samples.
References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Harteneck, C. (2005) Naunyn Schmiedebergs Arch Pharmacol. 371, 307. 6. Schmitz, C. et al. (2005) J.Biol.Chem. 280, 37763. 7. Aarts, M.M. and Tymianski, M. (2005) Neuroscientist 11, 116. 8. Aarts, M.M. and Tymianski, M. (2005) Pflugers Arch – Eur.J.Physiol. 451, 243.
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