|Reactivity||Human, Mouse, Rat|
|Calculated MW||134301 Da|
|Homology||Rat, mouse- 12/13 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily M member 4, hTRPM4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, LTrpC-4, LTrpC4, Melastatin-4, TRPM4, LTRPC4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 ofֲ human TRPM4 (Accession Q8TD43).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4. TRPM4 is expressed at relatively high levels in cardiac tissues. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPM4. The Anti-TRPM4 antibody (#AG1449) can be used in western blot and immunohistochemical applications. It has been designed to recognize TRPM4 from human, rat and mouse samples.
References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Kraft, R. and Harteneck, H. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204. 3. Huang, C-L. et al. (2004) J. Am. Soc. Nephrol. 15, 1690. 4. Vazquez, E. and Valverde, M.A. (2006) Semin. Cell Dev. Biol. 17, 607.
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