|Homology||Canis - 12/16 amino acid residues identical.|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1 (Accession O75560).ֲ Intracellular, C-terminus.Will not recognize rat or mouse TRPM1.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas. The TRPM family consist of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7. The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family. TRPM channels were suggested to play a role in tumourigenesis, proliferation and differentiation. Recently, TRPM1 was found to be down-regulated in a highly metastatic line. The loss of TRPM1 expression has been reported to be correlated to melanoma aggressiveness. Two alternative splice variants of TRPM1 were reported; short (TRPM1-S) and long (TRPM1-L). The TRPM1-S was described to be expressed at human melanocyte and mainly in metastasic melanoma cell lines. While TRPM1-L is expressed at murine CNS. Abgent is pleased to introduce a new antibody directed to the human TRPM1, Anti-Human TRPM1 antibody, to our growing line of TRP antibodies
References 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. 2. Vazquez, E. and Valverde, M.A. (2006) Semin. Cell Dev. Biol. 17, 607. 3. Kraft, R. and Harteneck, H. (2005) Pflugers Arch – Eur. J. Physiol. 451, 204. 4. Fang, D. and Setaluri, V. (2000) Biochem Biophys Res Commun. 279, 53.
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