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TRPV3 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - TRPV3 (extracellular) Antibody AG1451-025
    Western blot analysis of rat DRG (lanes 1 and 2) and rat brain (lanes 3 and 4) lysates:
    1, 3. Anti-TRPV3 (extracellular) antibody (#AG1451), (1:200).
    2, 4. Anti-TRPV3 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - TRPV3 (extracellular) Antibody AG1451-025
    Expression of TRPV3 in mouse spinal cord
    Immunohistochemical staining of mouse spinal cord using Anti-TRPV3 (extracellular) antibody (#AG1451). A. TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B. Motor neurons were stained with goat anti choline-acetyltransferase (red). In some motor neurons (A) TRPV3 was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C. Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead). Note the punctate pattern of TRPV3 on the surface of the soma.
  • ICC - TRPV3 (extracellular) Antibody AG1451-025
    Expression of TRPV3 in rat DRG primary culture
    Immunocytochemical staining of living rat dorsal root ganglion (DRG) primary culture using Anti-TRPV3 (extracellular) antibody (#AG1451), (1:50-1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Nuclear staining with the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NET8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 90636 Da
Homology Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.
Additional Information
Gene ID 162514
Other Names Transient receptor potential cation channel subfamily V member 3, TrpV3, Vanilloid receptor-like 3, VRL-3, TRPV3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)REEEAIPHPLALTHK, corresponding to amino acidֲ residues 464-478 of human TRPV3 (Accession Q8NET8). 1stֲ  extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3 According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7 TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human TRPV3. The Anti-TRPV3 (extracellular) (#AG1451) can be used for western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize TRPV3 from human, rat and mouse samples.


References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Gunthorpe, al. (2002) Trends. Pharmacol. Sci. 23, 183. 6. Tominaga, M. and Caterina, M.J. (2004) J.Neurobiol. 61, 3. 7. Voets, T. et al. (2004) Nature 430, 748. 8. Xiao, R. et al. (2008) J.Biol.Chem. 283, 6162.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1451-025
(40 western blots)
Availability: 2-3 weeks
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