|Reactivity||Human, Mouse, Rat|
|Calculated MW||94948 Da|
|Homology||Guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.|
|Other Names||Transient receptor potential cation channel subfamily V member 1, TrpV1, Capsaicin receptor, Osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid receptor type 1-like, Trpv1, Vr1, Vr1l|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Immunohistochemistry (IH): - Human uveal melanoma sections (1:200) (see Mergler, S. et al. (2014) in Product Citations). - Rat DRG sections (1:1000) (see Isensee, J. et al. (2014) in Product Citations). - Human skin sections (1:1000) (see Cheng, H.T. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel. The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3 TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.5,6 Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating. There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9
References 1. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183. 2. Cesare, P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 7658. 3. Kim, J. et al. (2003) Nature 424, 81. 4. Ahern, G. P. et al. (2003) J. Biol. Chem. 278, 30429. 5. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790. 6. Tominaga, M. et al. (1998) Neuron 21, 531. 7. Vlachova, V. et al. (2003) J. Neurosci. 23, 1340. 8. Agopyan, N. et al. (2004) Am. J. Physiol. 286, L563. 9. Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
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