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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRPC6 Antibody   

TRPC6 Antibody

Affinity purified polyclonal antibody

  • WB - TRPC6 Antibody AG1455-025
    Western blot analysis of  rat brain membranes:
    1. Anti-TRPC6 antibody (#AG1455), (1:200).
    2. Anti-TRPC6 antibody, preincubated with the control peptide antigen.
  • IHC - TRPC6 Antibody AG1455-025
    Expression of  TRPC6 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-TRPC6 antibody (#AG1455). A. TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti parvalbumin in the same brain section. C. Confocal merge of TRPC6 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61143
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 106681 Da
Homology Rat - identical; human - 15/16 amino acid residues identical.
Additional Information
Gene ID 22068
Other Names Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Transient receptor protein 6, TRP-6, Trpc6, Trp6, Trrp6
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143).ֲ ֲ Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Application Details Western blot analysis (WB): - Rat glomerular mesangial cells (HBZY-1) (1:1000) (see Qiu, G. and Ji, Z. (2013) in Product Citations). - Mouse glomeruli (see Kistler, A.D. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc6-/- mice. - Immortalized mouse podocytes (see Zhu, L. et al. (2013) in Product Citations). - Mouse platelets (also tested in trpc6-/- mice) (see Harper, M.T. et al. (2013) in Product Citations). - Human platelets (see Harper, M.T. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see Zhang, Y. et al.  (2013) in Product Citations). - HEK 293 cells expressing mouse TRPC6 (1:200) (see Shi, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Human kidney sections (see Kistler, A.D. et al. (2013) in Product Citations). - Rat kidney (see Sonneveld, R. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat mesenteric artery smooth muscle cells (1:100) (see Brueggemann, L.I. et al. (2014) in Product Citations). - Rat glomerular mesangial cells (HBZY-1) (1:1000) (see Qiu, G. and Ji, Z. (2013) in Product Citations). - HEK 293 cells expressing mouse TRPC6 (1:500) (see Shi, J. et al. (2013) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl,  50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1. 2. TRPC4 and TRPC5. 3. TRPC3, TRPC6, TRPC7. 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease.6,7


References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Winn. M.P. et al. (2005) Science, 308, 1801. 7. Reiser, J. et al. (2005) Nat. Genet. 37, 739.

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Cat# AG1455-025
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