|Reactivity||Human, Mouse, Rat|
|Calculated MW||106681 Da|
|Homology||Rat - identical; human - 15/16 amino acid residues identical.|
|Other Names||Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Transient receptor protein 6, TRP-6, Trpc6, Trp6, Trrp6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Application Details||Western blot analysis (WB): - Rat glomerular mesangial cells (HBZY-1) (1:1000) (see Qiu, G. and Ji, Z. (2013) in Product Citations). - Mouse glomeruli (see Kistler, A.D. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc6-/- mice. - Immortalized mouse podocytes (see Zhu, L. et al. (2013) in Product Citations). - Mouse platelets (also tested in trpc6-/- mice) (see Harper, M.T. et al. (2013) in Product Citations). - Human platelets (see Harper, M.T. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see Zhang, Y. et al. (2013) in Product Citations). - HEK 293 cells expressing mouse TRPC6 (1:200) (see Shi, J. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Human kidney sections (see Kistler, A.D. et al. (2013) in Product Citations). - Rat kidney (see Sonneveld, R. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat mesenteric artery smooth muscle cells (1:100) (see Brueggemann, L.I. et al. (2014) in Product Citations). - Rat glomerular mesangial cells (HBZY-1) (1:1000) (see Qiu, G. and Ji, Z. (2013) in Product Citations). - HEK 293 cells expressing mouse TRPC6 (1:500) (see Shi, J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||1 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1. 2. TRPC4 and TRPC5. 3. TRPC3, TRPC6, TRPC7. 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease.6,7
References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Winn. M.P. et al. (2005) Science, 308, 1801. 7. Reiser, J. et al. (2005) Nat. Genet. 37, 739.
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