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TRPC3 Antibody

Affinity purified polyclonal antibody

  • WB - TRPC3 Antibody AG1456-025
    Western blot analysis of rat heart membranes:
    1. Anti-TRPC3 antibody (#AG1456), (1:200).
    2. Anti-TRPC3 antibody, preincubated with the control peptide antigen.
  • IHC - TRPC3 Antibody AG1456-025
    Expression of TRPC3 in rat pituitary gland
    Immunohistochemical staining of rat pituitary gland paraffin embedded sections using Anti-TRPC3 antibody (#AG1456), (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
  • IHC - TRPC3 Antibody AG1456-025
    Expression of TRPC3 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-TRPC3 antibody (#AG1456) (A). Immunoreactivity appears in the molecular layer and in Purkinje cells (B).
  • IHC - TRPC3 Antibody AG1456-025
    Expression of TRPC3 in rat cerebellum
    Immunohistochemical staining of TRPC3 in rat cerebellum using Anti-TRPC3 antibody (#AG1456). A. TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with mouse anti-parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons. B. Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining. DAPI is used as the counterstain (blue).
  • IHC - TRPC3 Antibody AG1456-025
    Colocalization of TRPC6 and TRPC3 in rat cerebellum Immunohistochemical staining of rat cerebellum frozen section using guinea pig Anti-TRPC6 antibody (#AGP-002) and rabbit Anti-TRPC3 antibody (#AG1456). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates extensive co-localization. DAPI is used as the counterstain (blue).
  • ICC - TRPC3 Antibody AG1456-025
    Expression of TRPC3 in rat C6 brain glioma cells
    Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized rat C6 brain glioma cells. A. Staining using Anti-TRPC3 antibody (#AG1456), (1:500) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QZC1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 95672 Da
Homology Human, rat - 13/14 amino acid residues identical.
Additional Information
Gene ID 22065
Other Names Short transient receptor potential channel 3, TrpC3, Receptor-activated cation channel TRP3, Transient receptor protein 3, TRP-3, mTrp3, Trp-related protein 3, Trpc3, Trp3, Trrp3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1).ֲ ֲ Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Application Details Western blot analysis (WB): - Rat cortical astrocyte lysate (1:500) (see Roedding, A.S. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc3-/- mice. - Rat hippocampus lysate (1:2000) (see Ryu, H.J. et al. (2013) in Product Citations).   Immunoprecipitation (IP): - HEK-293 cells transfected with human TRPC3 (see Kwan, H.Y. et al. (2004) in Product Citations).   Immunohistochemistry (IH): - Mouse cerebellum (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc3-/- mice. - Rat brain free-floating sections (1:200) (see Ryu, H.J. et al. (2013) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7


References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Vazquez, G. et al. (2003) J. Biol.Chem. 278, 21649. 7. Venkatachalam, K. et al. (2003) J. Biol.Chem. 278, 29031.

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$ 375.00
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Cat# AG1456-025
(40 western blots)
Availability: 2-3 weeks
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