|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||95672 Da|
|Homology||Human, rat - 13/14 amino acid residues identical.|
|Other Names||Short transient receptor potential channel 3, TrpC3, Receptor-activated cation channel TRP3, Transient receptor protein 3, TRP-3, mTrp3, Trp-related protein 3, Trpc3, Trp3, Trrp3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Application Details||Western blot analysis (WB): - Rat cortical astrocyte lysate (1:500) (see Roedding, A.S. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc3-/- mice. - Rat hippocampus lysate (1:2000) (see Ryu, H.J. et al. (2013) in Product Citations). Immunoprecipitation (IP): - HEK-293 cells transfected with human TRPC3 (see Kwan, H.Y. et al. (2004) in Product Citations). Immunohistochemistry (IH): - Mouse cerebellum (see Feng, S. et al. (2013) in Product Citations). Also tested in Trpc3-/- mice. - Rat brain free-floating sections (1:200) (see Ryu, H.J. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7
References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Vazquez, G. et al. (2003) J. Biol.Chem. 278, 21649. 7. Venkatachalam, K. et al. (2003) J. Biol.Chem. 278, 29031.
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