|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||91212 Da|
|Homology||Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.|
|Other Names||Short transient receptor potential channel 1, TrpC1, Transient receptor protein 1, TRP-1, TRPC1, TRP1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995).ֲ ֲ Intracellular.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Application Details||Western blot analysis (WB): - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see Zhang, Y. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Human umbilical vein endothelial cells (HUVECs), (see Ahmmed, G.U. et al. (2004) in Product Citations). Immunocytochemistry (IC): - BAE-1 (Bovine aortic endothelium) cells (1:200) (see Antoniotti, S. et al. (2002) in Product Citations). - Human U373 MG cells (1:80) (see Barajas, M. et al. (2008) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) TRPC3, TRPC6, TRPC7 4) TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC1 was the first mammalian TRP protein that was reported to form an ion channel.2 It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet.1-3 The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.6
References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.
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