|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||250136 Da|
|Homology||Hamster, mouse - 16/17 amino acid residues identical; human -15/17 amino acid residues identical; chicken - 12/17 amino acid residues identical.|
|Other Names||Voltage-dependent L-type calcium channel subunit alpha-1D, Calcium channel, L type, alpha-1 polypeptide, isoform 2, Rat brain class D, RBD, Voltage-gated calcium channel subunit alpha Cav13, Cacna1d, Cach3, Cacn4, Cacnl1a2, Cchl1a2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DNKVTIDDYQEEAEDKD, corresponding to amino acid ֲ residues 859-875 of rat Cav1.3ֲ (Accession P27732).ֲ ֲ Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat primary submucosa cells (see Rehn, M. et al. (2013) in Product Citations). - Mouse mesenteric and pulmonary arteries (1:200) (see Ko, E.A. et al. (2013) in Product Citations). - Human SAN tissue (see Le Scouarnec, S. et al. (2008) in Product Citations). Immunoprecipitation (IP): - Rat cerebral cortex, thalamus, cerebellum, hippocampus, and spinal cord lysates (see Kim, S. et al. (2007) in Product Citations). Immunocytochemistry (IC): - Rat primary submucosa cells (1:50) (see Rehn, M. et al. (2013) in Product Citations). - Rat SCG and hippocampal pyramidal neurons (1:1000) (see Wheeler, D.G. et al. (2012) in Product Citations). - Mouse SAN cells (see Le Scouarnec, S. et al. (2008) in Product Citations). Immunohistochemistry (IH): - Rat intracardiac ganglia and stellate ganglia neurons (see Tu, H. et al. (2014) in Product Citations). - Mouse cochlea (see Pirone, A. et al. (2014) in Product Citations). - Mouse apical cochlear turns (1:75) (see Wong, A.B. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2 The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4 Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.
References 1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579. 2. IUPHAR 3. Mangoni, M.E. et al (2003) Proc. Natl. Acad. Sci.100, 5543. 4. Zhang, Z. et al. (2005) Circulation 112, 1936.
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