|Application ||WB, IHC, IP, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||262256 Da|
|Homology||Mouse - 15/17 amino acid residues identical; human 11/17 amino acid residues identical; rabbit - 11/17 amino acid residues identical.|
|Other Names||Voltage-dependent N-type calcium channel subunit alpha-1B, Brain calcium channel III, BIII, Calcium channel, L type, alpha-1 polypeptide isoform 5, Voltage-gated calcium channel subunit alpha Cav22, Cacna1b, Cach5, Cacnl1a5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat Cav2.2 (Accession Q02294). Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Application Details||Western blot analysis (WB): - Rat primary submucosa cells (see Rehn, M. et al. (2013) in Product Citations). - Mouse synaptosome (1:300) (see Saggu et al. (2013) in Product Citations). Immunoprecipitation (IP): - Transfected tsA201 cells (1:200) (see Marangoudakis, S. et al. (2012) in Product Citations). Immunohistochemistry (IH): - Rat retinal sections (1:1000) (see Sargoy, A. et al. (2013) in Product Citations). - Rat intracardiac ganglia and stellate ganglia neurons (see Tu, H. et al. (2014) in Product Citations). - Rat retinal sections (1:1000-1:1500) (see Liu, X. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat primary submucosa cells (1:100) (see Rehn, M. et al. (2013) in Product Citations). - NCI-H295R human adrenocortical cell line (H295R) (1:100) (see Aritomi, S. et al. (2011) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1 The voltage dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type. The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2 The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3 The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4 ω-Conotoxin GVIA (#C-300) is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 α1 subunit (α1B) and its action is only partially reversible.5,6
1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
2. Beuckmann, C.T. et al. (2003) J. Neurosci. 23, 6793.
3. Scheuber, A. et al. (2004) J. Neurosci. 24,10402.
4. Dascal, N. (2001) Trends Endocrinol. Metab. 12, 391.
5. McCleskey, E. W. et al. (1987) Proc. Natl. Acad.Sci. USA 84, 4327.
6. Feng, Z. P. et al. (2001) J. Biol. Chem. 276, 15728.
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