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Angiotensin II Receptor Type-2 (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Angiotensin II Receptor Type-2 (extracellular) Antibody AG1462-025
    Western blot analysis of mouse MS1 endothelial cells: 1. Anti-Angiotensin II Receptor Type-2 (extracellular) antibody, (AG1462), (1:400).
    2. Anti-Angiotensin II Receptor Type-2 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Angiotensin II Receptor Type-2 (extracellular) Antibody AG1462-025
    Western blot analysis of rat brain membranes: 1. Anti-Angiotensin II Receptor Type-2 (extracellular) antibody (AG1462), (1:500).
    2. Anti-Angiotensin II Receptor Type-2 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Angiotensin II Receptor Type-2 (extracellular) Antibody AG1462-025
    Expression of Angiotensin II Receptor Type-2 in rat brain Immunohistochemical staining of rat brain sections using Anti-Angiotensin Receptor Type-2 (extracellular) antibody (#AG1462). A. AT2 receptor expressing neurons (red) are scattered in the paraventricular nucleus of the hypothalamus (arrows), in the vicinity of the 3rd ventricle (3rd V). B. AT2 receptor expressing neurons in the supraoptic nucleus (arrows), adjacent to the optic nerve. Nissl is used as the counterstain (blue).
  • ICC - Angiotensin II Receptor Type-2 (extracellular) Antibody AG1462-025
    Expression of Angiotensin II-Receptor Type-2 in rat GH3 pituitary cells Immunocytochemical staining of live intact rat GH3 pituitary cells using Anti-Angiotensin II Receptor Type-2 (extracellular) antibody (#AG1462), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, LCI
Primary Accession P35351
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 41331 Da
Homology Mouse - identical.
Additional Information
Gene ID 24182
Other Names Type-2 angiotensin II receptor, Angiotensin II type-2 receptor, AT2, Agtr2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351).ֲ ֲ  Extracellular, N-terminal domain.Will not recognize human AT2 receptor.
Dilution WB~~1:200-1:2000
ICC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Mouse lung lysate (1:1000) (see Rodriguez-Machado, M.G. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II).   Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.   Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension.   Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as  SHP-1.   AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.

References

References 1. Mukoyama, M. et al. (1993) J. Biol. Chem. 268, 24539. 2.  De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415. 3. Carey, R.M. and Siragy, H.M. (2003) Endocr. Rev. 24, 261.

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Cat# AG1462-025
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