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Angiotensin II Receptor Type-1 (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Angiotensin II Receptor Type-1 (extracellular) Antibody AG1463-025
    Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: 1, 2. Anti-Angiotensin II Receptor Type-1 (extracellular) antibody (AG1463), (1:500). 3, 4. Anti-Angiotensin II Receptor Type-1 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Angiotensin II Receptor Type-1 (extracellular) Antibody AG1463-025
    Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: 1, 2. Anti-Angiotensin II Receptor Type-1 (extracellular) antibody (AG1463), (1:200). 3, 4. Anti-Angiotensin II Receptor Type-1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Angiotensin II Receptor Type-1 (extracellular) Antibody AG1463-025
    Expression of Angiotensin II Receptor Type-1 in mouse cerebellum Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) antibody (#AG1463). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B pannels reveals partial co-localization.
  • ICC - Angiotensin II Receptor Type-1 (extracellular) Antibody AG1463-025
    xpression of Angiotensin II Receptor Type-1 in rat C6 glioma cells Immunocytochemical staining of live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) antibody (#AG1463), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Live intact C6 cells.
  • FC - Angiotensin II Receptor Type-1 (extracellular) Antibody AG1463-025
    Indirect flow cytometry analysis of live intact human Jurkat T-cell leukemia cells:
    Black: Unstained cells + goat-anti-rabbit-FITC.
    Green: Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) antibody (#AG1463), (5 µg) + goat-anti-rabbit-FITC.
    Green: Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) antibody, (10 µg) + goat-anti-rabbit-FITC.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, LCI
Primary Accession P30556
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 41061 Da
Homology Mouse, rat, rabbit, dog - identical.
Additional Information
Gene ID 185
Other Names Type-1 angiotensin II receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1, AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556).   Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
ICC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Rat heart lysate (1:250) (see Wang, H. et al. (2013) in Product Citations). - Mouse lung lysate (1:1000) (see Rodriguez-Machado, M.G. et al. (2013) in Product Citations). - Sheep brain medulla lysate (1:200) (see Marshall, A.C. et al. (2013) in Product Citations). Indirect flow cytometry (IFC): - Mouse gastrocnemius muscle cells (1:100) (see Yoshida, T. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Mouse isolated myofibers (1:100) (see Yoshida, T. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.7 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Background

Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

References

1. Sasaki, K. et al. (1991) Nature 351, 230.
2. Murphy, T.J. et al. (1991) Nature 351, 233.
3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953.

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Cat# AG1463-025
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