Angiotensin II Receptor Type-1 (extracellular) Antibody
Affinity purified rabbit polyclonal antibody
|Application ||WB, IHC, FC, ICC, LCI|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||41061 Da|
|Homology||Mouse, rat, rabbit, dog - identical.|
|Other Names||Type-1 angiotensin II receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1, AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat heart lysate (1:250) (see Wang, H. et al. (2013) in Product Citations). - Mouse lung lysate (1:1000) (see Rodriguez-Machado, M.G. et al. (2013) in Product Citations). - Sheep brain medulla lysate (1:200) (see Marshall, A.C. et al. (2013) in Product Citations). Indirect flow cytometry (IFC): - Mouse gastrocnemius muscle cells (1:100) (see Yoshida, T. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Mouse isolated myofibers (1:100) (see Yoshida, T. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
1. Sasaki, K. et al. (1991) Nature 351, 230.
2. Murphy, T.J. et al. (1991) Nature 351, 233.
3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953.
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