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Connexin-45 Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - Connexin-45 Antibody AG1472-025
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Anti-Connexin-45antibody, (1:200).3, 4. Anti-Connexin-45 antibody, preincubated with the control peptide antigen.
  • WB - Connexin-45 Antibody AG1472-025
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1.Anti-Connexin-45antibody (#AG1472), (1:200).2. Anti-Connexin-45 antibody, preincubated with the control peptide antigen.
  • IHC - Connexin-45 Antibody AG1472-025
    Expression of Connexin-45 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 antibody (#AG1472), (1:400), followed by anti-rabbit-Cy3 antibody. Connexin-45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession A4GG66
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 45666 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 266706
Other Names Gap junction gamma-1 protein, Connexin-45, Cx45, Gap junction alpha-7 protein, Gjc1, Gja7
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Citations (0)

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Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

References

Teunissen B.E.,et al.DNA Cell Biol. 26:108-115(2007).
Mural R.J.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.

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Cat# AG1472-025
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