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Frizzled-2 (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - Frizzled-2 (extracellular) Antibody AG1489-025
    Western blot analysis of rat heart lysate:1. Anti-Frizzled-2 (extracellular) antibody (#AG1489), (1:200).2. Anti-Frizzled-2 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Frizzled-2 (extracellular) Antibody AG1489-025
    Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4):1-2.Anti-Frizzled-2 (extracellular) antibody (#AG1489), (1:200).3-4. Anti-Frizzled-2 (extracellular) antibody, preincubated with the control peptide antigen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q08464
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 63885 Da
Homology Human - identical; mouse - 13/14 amino acid residues identical.
Additional Information
Gene ID 64512
Other Names Frizzled-2, Fz-2, rFz2, Fzd2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Citations (0)

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Background

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activation by Wnt5A stimulates PKC activity via a G- protein-dependent mechanism.

References

Chan S.D.H.,et al.J. Biol. Chem. 267:25202-25207(1992).
Sheldahl L.C.,et al.Curr. Biol. 9:695-698(1999).

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$ 375.00
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Cat# AG1489-025
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