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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Vesicular Glutamate Transporter 2 Antibody   

Vesicular Glutamate Transporter 2 Antibody

Purified Rabbit Polyclonal Antibody

  • WB - Vesicular Glutamate Transporter 2 Antibody AG1494-025
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysate (lanes 3 and 6):1-3.Anti-Vesicular Glutamate Transporter 2 antibody (#AG1494), (1:400).4-6. Anti-Vesicular Glutamate Transporter 2 antibody, preincubated with the control peptide antigen.
  • IHC - Vesicular Glutamate Transporter 2 Antibody AG1494-025
    Expression of VGLUT2 in rat neocortex
    Immunohistochemical staining of rat frozen brain sections using Anti-Vesicular Glutamate Transporter 2 antibody (#AG1494), (1:400). VGLUT2 (red) is detected in pyramidal neurons (arrow). The same section was stained with DAPI (blue).
  • IHC - Vesicular Glutamate Transporter 2 Antibody AG1494-025
    Immuno-colocalization of VGLUT2 and P2X7 Receptor in rat spinal cord
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Vesicular Glutamate Transporter 2 antibody (#AG1494), (1:600) and Anti-P2X7 Receptor-ATTO-550 antibody (#APR-004-AO), (1:100). A. Vesicular Glutamate Transporter 2 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section labeled for P2X7 Receptor (orange). C. Merge of A and B demonstrates partial co-localization of VGLUT2 and P2X7 Receptor in dorsal horn and in lateral column (L. Col., arrow). Cell nuclei were stained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JI12
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 64576 Da
Homology Mouse - identical; human - 11/12 amino acid residues identical.
Additional Information
Gene ID 84487
Other Names Vesicular glutamate transporter 2, VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, Slc17a6, Dnpi, Vglut2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas. May also mediate the transport of inorganic phosphate.


Aihara Y.,et al.J. Neurochem. 74:2622-2625(2000).
Hayashi M.,et al.J. Biol. Chem. 276:43400-43406(2001).
Herzog E.,et al.J. Neurosci. 21:RC181-RC181(2001).
Takamori S.,et al.J. Neurosci. 21:RC182-RC182(2001).
Fremeau R.T. Jr.,et al.Neuron 31:247-260(2001).

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Cat# AG1494-025
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