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Human Orai1 (extracellular) Antibody

Purified Mouse Monoclonal Antibody

  • WB - Human Orai1 (extracellular) Antibody AG1515-025
    Western blot analysis using Anti-Human Orai1 (extracellular) mouse monoclonal antibody (#AG1515), (1:200):1. HEK-293 cells transfected with human Orai1.2. HEK-293 cells transfected with control vector.
  • ICC - Human Orai1 (extracellular) Antibody AG1515-025
    Expression of Orai1 in HEK-293 transfected cells
    Immunocytochemical staining of intact living HEK-293 cells expressing Orai1. A. Extracellular staining of HEK-293 cells transfected with Orai1 using Anti-Human Orai1 (extracellular) mouse monoclonal antibody (#AG1515), (red). B. Cells transfected with the empty vector show no Orai1 staining.
  • FC - Human Orai1 (extracellular) Antibody AG1515-025
    Indirect flow cytometry analysis of intact living Jurkat cells.
    ——Unstained cells.
    ——Cells + Anti-Human Orai1 (extracellular) mouse monoclonal antibody (#AG1515), (5-10 µg antibody/0.5-1 x 106 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96D31
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 32668 Da
Additional Information
Gene ID 84876
Other Names Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.


Ota T.,et al.Nat. Genet. 36:40-45(2004).
Feske S.,et al.J. Exp. Med. 202:651-662(2005).
Soboloff J.,et al.J. Biol. Chem. 281:20661-20665(2006).
Mercer J.C.,et al.J. Biol. Chem. 281:24979-24990(2006).
Peinelt C.,et al.Nat. Cell Biol. 8:771-773(2006).

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$ 175.00
$ 375.00
Cat# AG1515-025
Availability: 2-3 weeks
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