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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   KCa3.1 (SK4) (extracellular) Antibody   

KCa3.1 (SK4) (extracellular) Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Western blot analysis using Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517), (1:250):1. HEK cells transfected with control vector.2. HEK cells transfected with human KCa3.1.
  • WB - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Western blot analysis using Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517), (1:250):1. Mouse MS1 endothelial cells.2. Rat IEC-6 intestinal epithelial cells.3. Human LN-CaP prostate carcinoma cells.4. Human THP-1 acute monocytic leukemia cells.
  • IHC - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Immuno-colocalization of KCa3.1 and TRPC1 in mouse brain
    Immunohistochemical staining of mouse brain sections using Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517) and Anti-TRPC1 antibody (#ACC-010). TRPC1 staining (blue) is detected in neuroblasts and outside neuroblasts as well (in astrocytes). KCa3.1 (red) is detected in neuroblasts. Merged image demonstrates the partial co-localization between the two.Adapted from Turner, K.L. et al. (2013) Cereb. Cortex 24, 2388. with permission of Oxford University Press.
  • ICC - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Expression of KCa3.1 in live intact human LN-CaP prostate carcinoma cells
    Immunocytochemical staining of live intact human LN-CaP prostate carcinoma cells with Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517), (1:20), followed by goat-anti-mouse-DyLight-594 secondary antibody (red), (A). B. Live view of the cells. C. Merge of the two images.
  • FC - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Indirect flow cytometry analysis of live intact Raji (human Burkitts lymphoma B-cell) cell line:___ Cells + goat-anti-mouse-Cy5.___Cells + mouse IgM isotype control + goat-anti-mouse-Cy5.___ Cells + Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517), (1:20) + goat-anti-mouse-Cy5.
  • FC - KCa3.1 (SK4) (extracellular) Antibody AG1517-025
    Indirect flow cytometry analysis of live intact THP-1 (human acute monocytic leukemia cells) cell line:___ Cells + Goat-anti-mouse-Cy5.___ Cells + Mouse IgM isotype control+ Goat-anti-mouse-Cy5.___ Cells + Anti-KCa3.1 (SK4) (extracellular) mouse monoclonal antibody (#AG1517), (1:20) + Goat-anti-mouse-Cy5.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC
Primary Accession O15554
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW 47696 Da
Additional Information
Gene ID 3783
Other Names Intermediate conductance calcium-activated potassium channel protein 4, SK4, SKCa 4, SKCa4, IKCa1, IK1, KCa3.1, KCa4, Putative Gardos channel, KCNN4, IK1, IKCA1, KCA4, SK4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
Citations (0)

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Background

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.

References

Joiner W.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11013-11018(1997).
Ishii T.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11651-11656(1997).
Logsdon N.J.,et al.J. Biol. Chem. 272:32723-32726(1997).
Ghanshani S.,et al.Genomics 51:160-161(1998).
Ghanshani S.,et al.J. Biol. Chem. 275:37137-37149(2000).

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$ 300.00
$ 450.00
Cat# AG1517-025
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