|Application ||WB, IHC, FC, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47696 Da|
|Other Names||Intermediate conductance calcium-activated potassium channel protein 4, SK4, SKCa 4, SKCa4, IKCa1, IK1, KCa3.1, KCa4, Putative Gardos channel, KCNN4, IK1, IKCA1, KCA4, SK4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Reconstitution||25 µl or 100 µl double distilled water (DDW), depending on the sample size.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Formulation||Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
Joiner W.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11013-11018(1997).
Ishii T.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11651-11656(1997).
Logsdon N.J.,et al.J. Biol. Chem. 272:32723-32726(1997).
Ghanshani S.,et al.Genomics 51:160-161(1998).
Ghanshani S.,et al.J. Biol. Chem. 275:37137-37149(2000).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.