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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Vesicular Monoamine Transporter 2 Antibody   

Vesicular Monoamine Transporter 2 Antibody

Purified Rabbit Polyclonal Antibody

  • WB - Vesicular Monoamine Transporter 2 Antibody AG1525-025
    Western blot analysis of rat brain lysate (lanes 1 and 4); mouse brain lysate (lanes 2 and 5) and rat brain stem lysate (lanes 3 and 6):1-3.Anti-Vesicular Monoamine Transporter 2 antibody (#AG1525), (1:400).4-6. Anti-Vesicular Monoamine Transporter 2 antibody, preincubated with the control peptide antigen.
  • IHC - Vesicular Monoamine Transporter 2 Antibody AG1525-025
    Expression of VMAT2 in rat substancia nigra dopaminergic neurons
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Vesicular Monoamine Transporter 2 antibody (#AG1525), (1:1000). A. VMAT2 staining (red). B. tyrosine hydroxylase staining (green), a marker of dopaminergic neurons. C. Merge of A and B demonstrates VMAT2 expression in several dopaminergic neurons (arrows). Cell nuclei were stained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01827
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 55690 Da
Homology Rat, mouse, human - 12/13 amino acid residues identical.
Additional Information
Gene ID 25549
Other Names Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, Slc18a2, Svat, Vmat2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.


Liu Y.,et al.Cell 70:539-551(1992).
Erickson J.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:10993-10997(1992).
Dimaline R.,et al.J. Physiol. (Lond.) 490:249-256(1996).
Krantz D.E.,et al.J. Biol. Chem. 272:6752-6759(1997).

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Cat# AG1525-025
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