|Application ||WB, ICC|
|Calculated MW||53261 Da|
|Homology||Mouse - identical; human - 11/12 amino acid residues identical.|
|Other Names||Neuronal acetylcholine receptor subunit beta-3, Chrnb3, Acrb3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Format||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Storage After Reconstitution||-20ºC.|
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Provided below are standard protocols that you may find useful for product applications.
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Deneris E.S.,et al.J. Biol. Chem. 264:6268-6272(1989).
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