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Nicotinic Acetylcholine Receptor alpha9 Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - Nicotinic Acetylcholine Receptor alpha9 Antibody AG1533-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1-2.Anti-Nicotinic Acetylcholine Receptor α9 antibody (#AG1533), (1:400).3-4. Anti-Nicotinic Acetylcholine Receptor α9 antibody, preincubated with the control peptide antigen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P43144
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 54526 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 65024
Other Names Neuronal acetylcholine receptor subunit alpha-9, Nicotinic acetylcholine receptor subunit alpha-9, NACHR alpha-9, Chrna9, Acra9
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
Citations (0)

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Background

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.

References

Elgoyhen A.B.,et al.Cell 79:705-715(1994).
Groot-Kormelink P.J.,et al.Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
Katz E.,et al.Hear. Res. 141:117-128(2000).
Verbitsky M.,et al.Neuropharmacology 39:2515-2524(2000).
Elgoyhen A.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:3501-3506(2001).

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Cat# AG1533-025
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