|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||113054 Da|
|Homology||Mouse, dog, pig - identical; Human- 13/14 amino acid residues identical.|
|Other Names||Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, 18.104.22.168, Sodium pump subunit alpha-1, Atp1a1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Format||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Storage After Reconstitution||-20ºC.|
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Provided below are standard protocols that you may find useful for product applications.
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Shull G.E.,et al.Biochemistry 25:8125-8132(1986).
Hara Y.,et al.J. Biochem. 102:43-58(1987).
Herrera V.L.M.,et al.J. Cell Biol. 105:1855-1865(1987).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Schneider J.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6357-6361(1985).
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