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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Alpha 1 Na+/K+ ATPase Antibody   

Alpha 1 Na+/K+ ATPase Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - Alpha 1 Na+/K+ ATPase Antibody AG1534-025
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1, 2. Anti-Alpha 1 Na+/K+ATPaseantibody (#AG1534), (1:200).3, 4. Anti- Alpha 1 Na+/K+ ATPase antibody, preincubated with the control peptide antigen.Note: Samples should be heated up to 37C. Don’t boil samples.
  • WB - Alpha 1 Na+/K+ ATPase Antibody AG1534-025
    Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:1.Anti-Alpha 1 Na+/K+ATPaseantibody (#AG1534), (1:200).2. Anti- Alpha 1 Na+/K+ ATPase antibody, preincubated with the control peptide antigen.Note: Samples should be heated up to 37C. Don’t boil samples.
  • IHC - Alpha 1 Na+/K+ ATPase Antibody AG1534-025
    Expression of Alpha 1 Na+ K+ ATPase in rat kidney
    Immunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ATPaseantibody (#AG1534), (1:200). Alpha 1 Na+/K+ ATPase staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.
  • IHC - Alpha 1 Na+/K+ ATPase Antibody AG1534-025
    Expression of Alpha 1 Na+ K+ ATPase in mouse brain
    Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Alpha 1 Na+/K+ ATPase antibody (#AG1534), (1:400). Alpha 1 Na+/K+ ATPase staining (red) is apparent in the hippocampal dentate gyrus in neuronal soma (vertical arrows) and in astrocytes (horizontal arrow). DAPI (blue) is used to visualize cell nuclei.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P06685
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 113054 Da
Homology Mouse, dog, pig - identical; Human- 13/14 amino acid residues identical.
Additional Information
Gene ID 24211
Other Names Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, 3.6.3.9, Sodium pump subunit alpha-1, Atp1a1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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Background

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

References

Shull G.E.,et al.Biochemistry 25:8125-8132(1986).
Hara Y.,et al.J. Biochem. 102:43-58(1987).
Herrera V.L.M.,et al.J. Cell Biol. 105:1855-1865(1987).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Schneider J.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6357-6361(1985).

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Cat# AG1534-025
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