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>   home   >   Products   >   Primary Antibodies   >   KV1.4 (extracellular) Antibody   

KV1.4 (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - KV1.4 (extracellular) Antibody AG1573-025
    Western blot analysis of rat brain lysate:1.Anti-KV1.4 (extracellular) antibody (#AG1573), (1:400).2. Anti-KV1.4 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - KV1.4 (extracellular) Antibody AG1573-025
    Western blot analysis of mouse brain lysate:1.Anti-KV1.4 (extracellular) antibody (#AG1573), (1:200).2. Anti-KV1.4 (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - KV1.4 (extracellular) Antibody AG1573-025
    Expression of Kv1.4 in live intact rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.4 (extracellular) antibody (#AG1573), (1:25), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
  • IHC - KV1.4 (extracellular) Antibody AG1573-025
    Expression of KV1.4 in rat parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) antibody (#AG1573), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P15385
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 73390 Da
Homology Mouse - identical; human - 13/15 amino acid residues identical.
Additional Information
Gene ID 25469
Other Names Potassium voltage-gated channel subfamily A member 4, RCK4, RHK1, RK3, Voltage-gated potassium channel subunit Kv1.4, Kcna4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Citations (0)

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Background

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

References

Stuehmer W.,et al.EMBO J. 8:3235-3244(1989).
Tseng-Crank J.,et al.FEBS Lett. 268:63-68(1990).
Kim E.,et al.Nature 378:85-88(1995).
Aydar E.,et al.Neuron 34:399-410(2002).
Schulte U.,et al.Neuron 49:697-706(2006).

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$ 375.00
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Cat# AG1573-025
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