|Application ||WB, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||43501 Da|
|Homology||Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.|
|Other Names||P2X purinoceptor 4, P2X4, ATP receptor, Purinergic receptor, P2rx4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Format||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Storage After Reconstitution||-20ºC.|
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Provided below are standard protocols that you may find useful for product applications.
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Seguela P.,et al.J. Neurosci. 16:448-455(1996).
Bo X.,et al.FEBS Lett. 375:129-133(1995).
Wang C.-Z.,et al.Biochem. Biophys. Res. Commun. 220:196-202(1996).
Buell G.N.,et al.EMBO J. 15:55-62(1996).
Soto F.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:3684-3688(1996).
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