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Plexin-A1 (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

  • WB - Plexin-A1 (extracellular) Antibody AG1583-025
    Western blot analysis of rat (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1, 2. Anti-Plexin-A1 (extracellular) antibody (#AG1583), (1:400).3, 4. Anti-Plexin-A1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Plexin-A1 (extracellular) Antibody AG1583-025
    Immuno-colocalization of GluN1 and Plexin-A1 in rat olfactory bulb
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using guinea pig Anti-NMDA Receptor 1 (GluN1) (extracellular) antibody (#AGP-046), (1:600) and rabbit Anti-Plexin-A1 (extracellular) antibody (#AG1583), (1:400). A. GluN1 (green) is expressed in the glomeruli (horizontal arrows). B. Plexin A1 staining (red) in the same section, shows expression in the glomeruli (arrow) but also in other layers (vertical arrow) of the olfactory bulb. C. Merge of the two images show cases co-localization in the glomeruli vs. lack of co-localization in deeper layers of the olfactory bulb. Cell nuclei are stained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UIW2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 211067 Da
Homology Mouse, rat - 13/14 amino acid residues identical.
Additional Information
Gene ID 5361
Other Names Plexin-A1, Semaphorin receptor NOV, PLXNA1, NOV, PLXN1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down- stream signaling events in the cytoplasm (By similarity).


Muzny D.M.,et al.Nature 440:1194-1198(2006).
Maestrini E.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:674-678(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Wollscheid B.,et al.Nat. Biotechnol. 27:378-386(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1583-025
(40 western blots)
Availability: 2-3 weeks
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