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PICK1 Antibody

Purified Rabbit Polyclonal Antibody

  • WB - PICK1 Antibody AG1589-025
    Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate (lanes 1 and 3) and human HepG2 liver carcinoma cell lysate (lanes 2 and 4):1, 2. Anti-PICK1antibody (#AG1589), (1:200).3, 4. Anti-PICK1 antibody, preincubated with the control peptide antigen.
  • WB - PICK1 Antibody AG1589-025
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-PICK1antibody (#AG1589), (1:200).3, 4. Anti-PICK1 antibody, preincubated with the control peptide antigen.
  • IHC - PICK1 Antibody AG1589-025
    Expression of PICK1 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 antibody (#AG1589), (1:400), followed by anti-rabbit-Cy3 antibody. PICK1 staining (red in the hippocampal CA3 region is detected in neurons (arrows). Nuclei are stained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9EP80
Host Rabbit
Clonality Polyclonal
Calculated MW 46606 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 84591
Other Names PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein, Pick1, Prkcabp
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
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Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.


Xia J.,et al.Neuron 22:179-187(1999).
El Far O.,et al.Eur. J. Neurosci. 12:4215-4221(2000).
Lin W.-J.,et al.Biochem. J. 354:635-643(2001).
Chen W.,et al.Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
Dev K.K.,et al.Neuropharmacology 38:635-644(1999).

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1589-025
(40 western blots)
Availability: 2-3 weeks
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