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pan ASIC (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

     
  • WB - pan ASIC (extracellular) Antibody AG1595-025
    Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2.Anti-pan ASIC (extracellular) antibody (#AG1595), (1:500).3 -4. Anti-pan ASIC (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - pan ASIC (extracellular) Antibody AG1595-025
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):1-2.Anti-pan ASIC (extracellular) antibody (#AG1595), (1:500).3 -4. Anti-pan ASIC (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - pan ASIC (extracellular) Antibody AG1595-025
    Expression of ASIC channels in rat locus coeruleus
    Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-pan ASIC (extracellular) antibody (#AG1595), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. ASIC channel staining (green) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
  • ICC - pan ASIC (extracellular) Antibody AG1595-025
    Expression of ASIC in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-pan ASIC (extracellular) antibody (#AG1595), (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P55926
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 59641 Da
Homology ASIC1: Mouse, rat, human - identical.ASIC2: Mouse, rat, human - 14/15 amino acid residues identical.ASIC3: Mouse, rat, human - 12/15 amino acid residues identical.ASIC4: Mouse, rat - 12/15 amino acid residues identical; human - 11/15 amino acid residues identical.
Additional Information
Other Names Acid-sensing ion channel 1, ASIC1, Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2, Asic1, Accn2, Bnac2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
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Cat# AG1595-025
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