5-Hydroxytryptamine Receptor 2A (extracellular) Antibody
Purified Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB |
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Primary Accession | P14842 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 52850 Da |
Homology | Mouse - identical; human - 9/13 amino acid residues identical. |
Gene ID | 29595 |
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Other Names | 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A, Htr2a, Htr2 |
Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
Format | Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
Control Antigen Storage After Reconstitution | -20ºC. |

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Provided below are standard protocols that you may find useful for product applications.
Background
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
References
Julius D.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:928-932(1990).
Pritchett D.B.,et al.EMBO J. 7:4135-4140(1988).
Liu J.,et al.Genomics 11:231-234(1991).
Fiorica-Howells E.,et al.Am. J. Physiol. 282:G877-G893(2002).

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