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>   home   >   Products   >   Primary Antibodies   >   5-Hydroxytryptamine Receptor 2B (extracellular) Antibody   

5-Hydroxytryptamine Receptor 2B (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

  • WB - 5-Hydroxytryptamine Receptor 2B (extracellular) Antibody AG1597-025
    Western blot analysis of mouse brain (lanes 1 and 5), rat brain (lanes 2 and 6), rat uterus (lanes 3 and 7) and SH-SY5Y cells (lanes 4 and 8) lysates:1-4.Anti-5-Hydroxytryptamine Receptor 2B (extracellular) antibody (#AG1597), (1:200).5-8. Anti-5-Hydroxytryptamine Receptor 2B (extracellular) antibody preincubated with the control peptide antigen.
  • IHC - 5-Hydroxytryptamine Receptor 2B (extracellular) Antibody AG1597-025
    Expression of 5-Hydroxytryptamine Receptor 2B in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-5-Hydroxytryptamine Receptor 2B (extracellular) antibody (#AG1597) (1:400). A. 5-HT2B staining (red), appears in neurons of the Purkinje layer (vertical arrows) and in the molecular layer (horizontal arrow). B. Nuclear staining using DAPI as the counterstaining (blue). C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30994
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 53652 Da
Homology Mouse - identical, human - 11/15 amino acid residues identical. 
Additional Information
Gene ID 29581
Other Names 5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, 5-HT-2F, Serotonin receptor 2B, Stomach fundus serotonin receptor, Htr2b, Srl
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Citations (0)

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G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down- stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5- hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity). May play a role in the perception of pain.


Foguet M.,et al.EMBO J. 11:3481-3487(1992).
Kursar J.D.,et al.Mol. Pharmacol. 42:549-557(1992).
Urtikova N.,et al.Pain 153:1320-1331(2012).

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1597-025
(40 western blots)
Availability: 2-3 weeks
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