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VPAC2 (extracellular) Antibody

Purified Rabbit Polyclonal Antibody

  • WB - VPAC2 (extracellular) Antibody AG1604-025
    Western blot analysis of rat brain lysate (lanes 1 and 4); mouse brain lysate (lanes 2 and 5) and human Malme-3M melanoma cell lysate (lanes 3 and 6):1-3.Anti-VPAC2 (extracellular) antibody (#AG1604), (1:600).4-6. Anti-VPAC2 (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - VPAC2 (extracellular) Antibody AG1604-025
    Expression of VPAC2 in rat PC12 cells
    Immunocytochemical staining of live intact rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells with Anti-VPAC2 (extracellular) antibody (#AG1604), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
  • FC - VPAC2 (extracellular) Antibody AG1604-025
    Indirect flow cytometry analysis of live intact human Jurkat cell line:
    ——Cells + goat-anti-rabbit-DyLight-488.
    ——Cells + Anti-VPAC2 (extracellular) antibody (#AG1604), (1:15) + goat-anti-rabbit-DyLight-488.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41587
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 49479 Da
Homology Mouse, rat - identical.
Additional Information
Gene ID 7434
Other Names Vasoactive intestinal polypeptide receptor 2, VIP-R-2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R-3, PACAP-R3, VPAC2, VIPR2, VIP2R
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Format Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Storage After Reconstitution-20ºC.
Research Areas
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This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.


Svoboda M.,et al.Biochem. Biophys. Res. Commun. 205:1617-1624(1994).
Mackay M.,et al.Genomics 37:345-353(1996).
Wei Y.,et al.J. Neuroendocrinol. 8:811-817(1996).
Lutz E.M.,et al.FEBS Lett. 458:197-203(1999).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1604-025
(40 western blots)
Availability: 2-3 weeks
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