EBV Early Antigens (Epstein Barr Virus Ea) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, IP|
|Target/Specificity||Affinity purified early antigen polypeptides from induced Raji cells precipitated with African Burkitt’s lymphoma sera|
|Application Note||Immunofluorescence : 0.5-1.0 µg/ml |
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) : 0.5-1.0 µg/ml for 30 minutes at RT.
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||EBV Early Antigens (Epstein Barr Virus Ea) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness. The EBV-induced early antigens (Ea) are among several antigen complexes that have been identified in EBV-infected cells. The Ea complex is composed of diffuse (Ea-D) and restricted (Ea-R) components. The activity of Ea-D is suppressed during latent infection. BMRF1, the gene that encodes for Ea-D, is closely associated with the gene encoding for EBV DNA polymerase, and Ea-D is essential for the activity of this polymerase. Ea-D forms a complex with EBV DNase and, together, they may play a role in viral replication.
1. Epstein, Alan L.: Immunobiochemical characterization with monoclonal antibodies of Epstein-Barr virus-associated early antigens in chemically induced cells. J. Virology 50:372-379, 1984.
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