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>   home   >   Products   >   Primary Antibodies   >    Mitochondria (Marker for Human Cells, Granular RCC & Salivary Tumors) Antibody - With BSA and Azide   

Mitochondria (Marker for Human Cells, Granular RCC & Salivary Tumors) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Predicted Human
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names 113-1
Calculated MW 60kDa
Additional Information
Target/Specificity Semi-purified mitochondrial preparation
Application Note Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.25-0.5ug/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Mitochondria (Marker for Human Cells, Granular RCC & Salivary Tumors) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Abundant granular eosinophilic cytoplasm is a common feature of renal oncocytoma, chromophobe renal cell carcinoma, eosinophilic variant of papillary renal cell carcinoma, and the granular variant of clear cell renal cell carcinoma (RCC). The chief reason for their distinction from one another is the difference in their biologic behavior. However, precise characterization may be difficult in some cases because of overlapping morphologic features. Immunostaining pattern with anti-mitochondrial MAb 113-1 has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Moreover, Salivary gland tumors usually show great variability both in their morphopathology as well as their clinical behavior. One study highlights the usefulness of 113-1 MAb to facilitate the classification of salivary tumors, an aspect that may sometimes have not only diagnostic implications, but also prognostic.


1. Epstein, Alan L. and Clevenger, Charles V. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, Ed. CRC Press, Boca Raton, FL, pp. 117-137, 1985.
2. Tickoo SK, Amin MB, Linden MD, Lee MW, Zarbo RJ. Antimitochondrial antibody (113-1) in the differential diagnosis of granular renal cell tumors. Am J Surg Pathol. 1997;21(8):922-30.
3. Vera-Sempere F, Vera-Sirera B. Usefulness of antimitochondrial antibody 113-1 in diagnosis and classification of salivary gland tumours with oncocytic differentiation. Acta Otorrinolaringol Esp. 2011;62(1):1-9.

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$ 124.00
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$ 454.00
Cat# AH10006
(40 western blots)
Availability: 2-3 days
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